DNA of Drosophila melanogaster contains 5-methylcytosine
AUTOR(ES)
Gowher, Humaira
FONTE
Oxford University Press
RESUMO
It is commonly accepted that the DNA of Drosophila melanogaster does not contain 5-methylcytosine, which is essential in the development of most eukaryotes. We have developed a new, highly specific and sensitive assay to detect the presence of 5-methylcytosine in genomic DNA. The DNA is degraded to nucleosides, 5-methylcytosine purified by HPLC and, for detection by 1D- and 2D-TLC, radiolabeled using deoxynucleoside kinase and [γ-32P]ATP. Using this assay, we show here that 5-methylcytosine occurs in the DNA of D.melanogaster at a level of ∼1 in 1000–2000 cytosine residues in adult flies. DNA methylation is detectable in all stages of D.melanogaster development.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=305887Documentos Relacionados
- Estimation of the amount of 5-methylcytosine in Drosophila melanogaster DNA by amplified ELISA and photoacoustic spectroscopy.
- Detection of 5-methylcytosine in DNA sequences.
- Oxidative damage to 5-methylcytosine in DNA.
- The 5-methylcytosine content of DNA from human tumors.
- Type III 5-methylcytosine modification of DNA in Neisseria gonorrhoeae.