DNA methylation modulates H19 and IGF2 expression in porcine female eye
AUTOR(ES)
Wang, Dongxu, Wang, Guodong, Yang, Hao, Liu, Haibo, Li, Cuie, Li, Xiaolan, Lin, Chao, Song, Yuning, Li, Zhanjun, Liu, Dianfeng
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
06/03/2017
RESUMO
Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.
Documentos Relacionados
- Igf2 imprinting does not require its own DNA methylation or H19 RNA
- Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2
- Loss of the maternal H19 gene induces changes in Igf2 methylation in both cis and trans
- H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19
- Enhancer competition between H19 and Igf2 does not mediate their imprinting