DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy
Li, Deming, Fan, Jinyu, Li, Ziwei, Xu, Cunshuan
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF was down-regulated in total during liver regeneration. We think that the rat EGF promoter region is regulated by variation in DNA methylation during liver regeneration.
- The control of liver regeneration after partial hepatectomy in the rat.
- (2'-5')Oligoadenylate in rat liver: modulation after partial hepatectomy.
- Influence of pulsed electromagnetic fields on regenerating rat liver after partial hepatectomy.
- Gene expression during the priming phase of liver regeneration after partial hepatectomy in mice
- Timing of hepatocyte entry into DNA synthesis after partial hepatectomy is cell autonomous