DNA affinity capture and protein profiling by SELDI-TOF mass spectrometry: effect of DNA methylation
AUTOR(ES)
Bane, Thomas K.
FONTE
Oxford University Press
RESUMO
We report here that surface enhanced laser desorption/ionization–time of flight (SELDI-TOF) mass spectrometry, as performed on a Ciphergen Biosystems ProteinChip System, can be used in conjunction with DNA affinity capture (DACA) to study specific DNA–protein binding. Using DNA molecules bound to a surface, sequence-specific interactions can be detected as demonstrated by a mutation affecting the binding profile for TBP, a transcription factor. Also, a comparison between methylated and unmethylated promoter-containing DNA fragments shows numerous binding profile differences over a mass range extending to >60 kDa. The binding of several proteins is inhibited by methylation of the DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=135770Documentos Relacionados
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