Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping
AUTOR(ES)
Iturriza-Gómara, Miren
FONTE
American Society for Microbiology
RESUMO
A degenerate version (1T1-D) of the rotavirus P[8]-specific primer (1T-1) allowed strains previously untypeable due to the accumulation of point mutations at the primer binding site to be P typed by reverse transcription-PCR. Sequencing of the cDNA followed by sequence alignment and phylogenetic analysis identified lineages and sublineages within the rotavirus P[8] types, while the use of 1T-1 or 1T-1D primers did not yield viral clusters in any particular lineage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=86240Documentos Relacionados
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