Diversidade molecular do envelope e carga proviral do vírus linfotrópico de células T humanas tipo 2 (HTLV-2) em amostras indeterminadas pelo teste Western-Blot / Molecular diversity of the envelope and proviral load cell lymphotropic virus human T-cell type 2 (HTLV-2) in the test samples indeterminate Western-Blot

AUTOR(ES)

Ingrid Olah do Nascimento

DATA DE PUBLICAÇÃO

2010

RESUMO

Despite the human T-cell lymphotropic virus type 2 (HTLV-2) is considered low pathogenic, the serological diagnosis is important for counseling and monitoring. The most used confirmatory tests are Western Blot (WB) and PCR. However, in high-risk populations, about 50% of the indeterminate WB results when tested by PCR, in our study, confirmed the presence of HTLV-2. A hypothesis for the insensitivity of the WB used in Brazil would be the use of recombinant proteins from strains that do not circulate in our country. Another possibility is the level of immunosuppression, which may lead to lower production of anti-HTLV-2 circulating. The viral load of HTLV-2 can also be a factor of little stimulation of the immune system, resulting low amount of antibodies. This study tested the three hypothesis and we showed that high homology in the region of immunogenic viral envelope when aligned with the sequence of amino acids of the protein K55 (HTLV-2) of WB kit, the proviral load and immunessupression state were not important for inconclusive WB profiles.

ASSUNTO(S)

viral load reação em cadeia da polimerase polymerase chain reaction vírus linfotrópico t humano human t-cell lymphotropic virus type 2 (htlv-2) carga viral indeterminate wb wb indeterminado

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