Distribution, detection of enterotoxigenic strains and antimicrobial drug susceptibility patterns of Bacteroides fragilis group in diarrheic and non-diarrheic feces from Brazilian infants
AUTOR(ES)
Ferreira, Débora Paula, Silva, Vânia Lúcia, Guimarães, Danielle Aparecida, Coelho, Cíntia Marques, Zauli, Danielle Alves Gomes, Farias, Luiz Macêdo, Carvalho, Maria Auxiliadora Roque, Diniz, Claudio Galuppo
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2010-10
RESUMO
Despite the importance of gastrointestinal diseases and their global distribution, affecting millions of individuals around the world, the role and antimicrobial susceptibility patterns of anaerobic bacteria such as those in the Bacteroides fragilis group (BFG) are still unclear in young children. This study investigated the occurrence and distribution of species in the BFG and enterotoxigenic strains in the fecal microbiota of children and their antimicrobial susceptibility patterns. Diarrheic (n=110) and non-diarrheic (n=65) fecal samples from children aged 0-5 years old were evaluated. BFG strains were isolated and identified by conventional biochemical, physiological and molecular approaches. Alternatively, bacteria and enterotoxigenic strains were detected directly from feces by molecular biology. Antimicrobial drug susceptibility patterns were determined by the agar dilution method according to the guidelines for isolated bacteria. BFG was detected in 64.3% of the fecal samples (55% diarrheic and 80.4% non-diarrheic), and 4.6% were enterotoxigenic. Antimicrobial resistance was observed against ampicillin, ampicillin/sulbactam, piperacillin/tazobactam, meropenem, ceftriaxone, clindamycin and chloramphenicol. The data show that these bacteria are prevalent in fecal microbiota at higher levels in healthy children. The molecular methodology was more effective in identifying the B. fragilis group when compared to the biochemical and physiological techniques. The observation of high resistance levels stimulates thoughts about the indiscriminate use of antimicrobial drugs in early infancy. Further quantitative studies are needed to gain a better understanding of the role of these bacteria in acute diarrhea in children.
Documentos Relacionados
- Virulence factors and phylotyping of Escherichia coli isolated from non-diarrheic and diarrheic water buffalo calves
- Seasonal variation on the presence of adenoviruses in stools from non-diarrheic patients
- Bacteremia Due to Bacteroides fragilis Group: Distribution of Species, β-Lactamase Production, and Antimicrobial Susceptibility Patterns
- Isolation of Bacteroides fragilis from the feces of diarrheic calves and lambs.
- Survey of Bacteroides fragilis group susceptibility patterns in Canada.