Distribuição de MT1-MMP na região odontogenica de incisivos inferiores de ratos em condições funcionais de erupção normal e alterada : estudo imunohistoquimico / MT1-MMP distribuiton in the odontogenic region of lower incisors in rats with normal and altered eruption : an immunohistochemical study

AUTOR(ES)

Nadia Fayez Omar

DATA DE PUBLICAÇÃO

2008

RESUMO

Matrix metalloproteinases (MMPs) are a growing family of endopeptidases that are capable of degrading many structural components of the extracellular matrix, including collagen. MMPs on the dental follicle may be related with remodelation that occurs in this tissue during dental eruption. MT1-MMP has been considered an important membrane-type matrix metalloproteinase involved in tooth and root development, in alveolar bone formation, and in the dental eruption process. The aim of this investigation was to verify the distribution of MT1-MMP, using immunohistochemical technique, in the dental follicle of odontogenic region of lower incisors of rats, with normal and altered eruption conditions. Male adult Lewis rats were used. They were separated in 3 groups: Normalfunctional teeth group - teeth with normal eruption condition; hyper- and hypofunctional teeth group - the left lower incisors were cut at the level of the interdental papilla each two days, keeping the teeth out of occlusion (hypofunctional teeth) and the contralateral right incisors remained with overload (hyperfunctional teeth); Restrain teeth group, the left lower incisors received a restrain to impede the eruption process. After fourteen days the animals were anesthetized and sacrified by perfusion through the heart, the hemimandibles were removed and fixed, the fragments corresponding to the odontogenic region were removed. After descalcification in 10% EDTA they were embedded in paraffin. The sections were submitted to the immunohistochemical technique. MT1-MMP was distributed on the dental follicle area in all groups. In the normalfunctional teeth group the staining intensity was moderated, presenting a more concentrated distribution near the alveolar bone. The hyperfunctional teeth group showed staining intensity and distribution similar to the normalfunctional teeth group. The hypofunctional teeth group presented strong staining intensity with distribution extended to dental papilla. In the restrain teeth group the staining intensity was weak with diffuse distribution to all dental follicle area. The results suggest that the altered function condition eruption (hypo-, hyper-, and restrain tooth) may modify the MT1-MMP expression in rat incisor odontogenic region, demonstrating that this metalloproteinase may have an important role in the process of dental eruption

ASSUNTO(S)

immunohistochemistry metaloproteinases de matriz imunohistoquimica dentes - erupção matrix metalloproteinases tooth eruption

Documentos Relacionados

• Distribuição do EGF, EGFr, TGF- beta 1, IL-1 alfa e CSF-1 no periodonto de incisivos inferiores de ratos, em condições funcionais normal e alterada. Estudo imunohistoquimico
• Efeito da radioterapia na taxa de erupção e na morfologia da região odontogênica dos incisivos de rato
• MT1-MMP, MMP-2, MMP-9, TIMP-1,-2,-3 and the cell proliferation in the odontogenic region of the incisor tooth of adult rats : effects of doxycycline in the accelerated cell proliferation condition
• Expression of type I collagen, MMP-2, MMP-8, MMP-14 and TIMPS in the periodontal ligament of rat incisors in normal and altered functional conditions
• Imunolocalização de CSF-1, OPG e RANKL no periodonto relacionado ao esmalte de incisivos de ratos em erupção normal e contida