Disponibilidade de fósforo e resposta do capim marandu à adubação fosfatada em solos do Paraná

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Soybean (Glycine max (L. ) Merrill has been considered the main available source of vegetable protein and its culture has a worldwide relevance. Brazil is the second biggest producer and wordwide exporter. The high socioeconomic importace of soybean is mainly attributed to its much favorable combination of high protein and oil contents together with appropriate seed yield. Although soybean is considered a high quality source of oil and protein for food and feed, the soybean seeds contain at least three lipoxygenase isozymes, lipoxygenases 1, 2, and 3 (L1, L2, and L3). These isozymes are responsible or he production of unpleasant grassy and beany flavors in soybean seeds that have limited the development of soybean protein products for human consumption. Flavor improvement of soybean has been achieved through heat treatment, extraction with organic solvents or decomposition of beany flavor by aldehyde dehydrogenase. However, these treatments are expensive and not entirely satisfactory for food materials, because it may considerably decrease protein solubility. The genetic elimination of lipoxygenase (Lox 1, Lox 2 and Lox 3) enzymes from soybean seeds is a way to overcome the problems associated with the undesirable beany flavor of soybean seeds and genetic elimination of this flavor can be accelerated by using of molecular markers linked to Lox. The objective of this study was to map the L1, L2 and L3 loci and identify any markers to achieve in marker-assisted selection of plants lacking the three lipoxygenases in the seeds. A frame map based on simple sequence repeat (SSR) markers was constructed using a F2 population of BR 36 X BRS 213. To the segregation analysis of F2 was considered only two genes, due to the strong link between L1 and L2 loci. It was considered two genes segregating , due to the strong link between L1 and L2 loci. The results were confirmed by the chi-square test values, when the segregation was not statistically significant, occurring in accordance with the waited segregation and disposal 9:3:3:1 (9 - L1, L2 and L3 presence; 3 - L1 and L2 absence; 3 - L3 absence; and 1 - L1, L2 and L3 absence). When the genes were considered separately the segregation also occurred in waited pattern 3:1 disposal (3 - L1, L2 and L3 presence; 1 - L1 and L2 or L3 absence). Although the parental lines showed polymorphism for the tested markers for Lox 1 and 2 the F2 population did not presented polymorphism. Eight polymorphic markers were found for Lox 3 in the linkage group E and M. Based on the results of linkage analysis between Lox3 and the SSR markers, Lox 3 was found to be positioned on Linkage group E. Although markers from linkage group M had presented polymorphism they remained unlinked to locus L3. Regarding this, we can say that the locus responsible for the control of the isozyme Lox 3 is placed on the E link group. The Satt 212 marker (SSR) was integrated into the frame map obtained for locus L3 defined from the F2 population. This marker is 24.1 cM from locus Lox 3.

ASSUNTO(S)

phosphorus in soils plants plantas - efeito do fósforo solos - teor de fósforo capim marandu effect of phosphorus on

ACESSO AO ARTIGO

Documentos Relacionados