Differentiation of Gram-Negative, Nonfermentative Bacteria Isolated from Biofilters on the Basis of Fatty Acid Composition, Quinone System, and Physiological Reaction Profiles
AUTOR(ES)
Lipski, André
RESUMO
Gram-negative, nonfermentative bacteria isolated from biofilters for off-gas treatment of animal-rendering-plant emissions were differentiated by whole-cell fatty acid analysis, quinone analysis, and numerical taxonomy based on their physiological reaction profiles. The last system consisted of 60 physiological tests and was arranged as a microtest system on microtitration plates. Based on fatty acid analyses, 31 isolates were separated into six clusters and five single-member clusters. The isolates of two clusters were identified as Alcaligenes faecalis and Pseudomonas diminuta. The remaining nine clusters were characterized by their fatty acid profiles, quinone systems, and physiological reaction profiles. Clusters resulting from fatty acid analyses were compared with those resulting from physiological reaction profiles. Six clusters could be confirmed this way. The efficiency of the physiological test system was increased by the prearrangement of the isolates according to their quinone type.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=195726Documentos Relacionados
- Characterization and identification of gram-negative, nonfermentative bacteria.
- Numerical taxonomy of gram-negative, nonmotile, nonfermentative bacteria isolated during chilled storage of lamb carcasses.
- CDC Group O-3: Phenotypic Characteristics, Fatty Acid Composition, Isoprenoid Quinone Content, and In Vitro Antimicrobic Susceptibilities of an Unusual Gram-Negative Bacterium Isolated from Clinical Specimens
- Rapid method for identification of gram-negative, nonfermentative bacilli.
- Identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system.