Differentiation of Bacillus anthracis from Bacillus cereus by gas chromatographic whole-cell fatty acid analysis.

AUTOR(ES)

Lawrence, D

RESUMO

Three strains of Bacillus anthracis and seven strains of Bacillus cereus were grown on complex medium and on synthetic medium. Gas chromatographic analysis of whole-cell fatty acids of strains grown on complex medium gave nearly identical fatty acid patterns. Fatty acid patterns of strains grown on synthetic medium showed a high content of branched-chain fatty acids. Significant differences between the fatty acid patterns of the two species were found. Odd iso/anteiso fatty acid ratios were about equal in B. anthracis strains, whereas in B. cereus strains the fractions of iso acids were at least twice as high as the fractions of anteiso acids. The method described herein is used in our diagnostic laboratory to help differentiate between these two species.

Documentos Relacionados

• Gas chromatographic characterization of mycobacteria: analysis of fatty acids and trifluoroacetylated whole-cell methanolysates.
• Characterization of Clostridia by Gas Chromatography: Differentiation of Species by Trimethylsilyl Derivatives of Whole-Cell Hydrolysates
• Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids.
• Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus.
• Differentiation of Lactococcus lactis and Lactococcus garvieae from humans by comparison of whole-cell protein patterns.