Differential expression of nicotinic acetylcholine receptor genes in innervated and denervated chicken muscle.
AUTOR(ES)
Moss, S J
RESUMO
We have studied the mRNAs encoding all four subunits of the acetylcholine receptor in 13-day embryonic, innervated and denervated chicken pectoral muscle. In all three states the transcript sizes of the alpha, beta-, gamma- and delta-subunit mRNAs were approximately 3.2, 2.8, 1.8 and 1.9 kb respectively. Denervation was found to result in a large increase in the steady-state levels of each mRNA compared with those in innervated muscle. This increase was 8- to 9-fold for the beta and delta subunits and approximately 12-fold for the alpha subunit. The evidence obtained shows a coordinate regulation of the acetylcholine receptor genes in response to denervation. Interestingly, no gamma-subunit transcript was detected in innervated muscle, while significant levels were detected in embryonic and denervated tissue. This evidence suggests that there exists in a non-mammalian muscle acetylcholine receptor an additional subunit, analogous to the bovine epsilon subunit, and shows that the extra-junctional receptor of adult denervated muscle is produced by the same set of mRNAs which produce the major form of the receptor in embryonic muscle.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553869Documentos Relacionados
- Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.
- Differential expression of human nicotinic acetylcholine receptor alpha subunit variants in muscle and non-muscle tissues.
- Differences in the interaction of acetylcholine receptor antibodies with receptor from normal, denervated and myasthenic human muscle.
- Experimental myasthenia in Balb/c mice immunized with rat acetylcholine receptor from rat denervated muscle.
- Acetylcholine receptors in singly and multiply innervated skeletal muscle fibres of the chicken during development.