Differential Expression of Desulfovibrio vulgaris Genes in Response to Cu(II) and Hg(II) Toxicity
AUTOR(ES)
Chang, In Seop
FONTE
American Society for Microbiology
RESUMO
The response of Desulfovibrio vulgaris to Cu(II) and Hg(II) was characterized. Both metals increased the lag phase, and Cu(II) reduced cell yield at concentrations as low as 50 μM. mRNA expression was analyzed using random arbitrarily primed PCR, differential display, and quantitative PCR. Both Cu(II) and Hg(II) (50 μM) caused upregulation of mRNA expression for an ATP binding protein (ORF2004) and an ATPase (ORF856) with four- to sixfold increases for Hg(II) and 1.4- to 3-fold increases with Cu(II). These results suggest that D. vulgaris uses an ATP-dependent mechanism for adapting to toxic metals in the environment.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368381Documentos Relacionados
- SUPRAMOLECULARIDADE DE TRIAZENOS ASSIMÉTRICOS DISSUBSTITUÍDOS 4-(ETOXICARBONILFENIL)-2-(HALOFENIL) E COMPLEXOS DE Ni(II), Cu(II) E Hg(II)
- SÍNTESE E CARACTERIZAÇÃO DE TRIAZENOS E COMPLEXOS COM Hg(II) INCLUINDO ARRANJOS SUPRAMOLECULARES
- Bioluminescent sensors for detection of bioavailable Hg(II) in the environment.
- merA gene expression in aquatic environments measured by mRNA production and Hg(II) volatilization.
- Interactions of Hg(II) ions with DNA as revealed by CD measurements.