Differential expression analysis of ID2, PRELP and SMOC2 genes in ectopic and eutopic endometrium in women with and without endometriosis in the proliferative phase of the menstrual cycle. / Análise da expressão diferencial dos genes ID2, PRELP e SMOC2 em endométrio ectópico e eutópico de mulheres com e sem endometriose na fase proliferativa do ciclo menstrual

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

07/10/2011

RESUMO

Endometriosis is a complex disease and its etiology is multifactorial, characterized by the presence of endometrial tissue outside the uterine cavity especially in the pelvic peritoneum and ovaries, involving genetic predisposition, environmental factors, anatomical, endocrine and immunological changes. It affects 10 to 15% of women of reproductive age and 35 to 50% of women with infertility, pelvic pain or both. Despite being one of the most studied diseases in gynecology, its etiology remains unclear and there are several current theories to explain it. The study of genes that regulate the processes involved somehow with endometriosis as ID2 (cell proliferation), PRELP (extracellular matrix) and SMOC2 (angiogenesis) may help clarify its development. The aim of this study was to analyze the gene expression of these genes in tissue samples from 20 women paired with 10 endometrial tissue and 10 peritoneal endometriotic lesions 10 endometrial tissue and 10 ovarian endometriotic lesions in proliferative phase of the menstrual cycle. Sixteen samples of endometrium of women without endometriosis, in the same phase of the menstrual cycle were collected to control (C). The study was conducted through molecular biology techniques such as reverse transcription (RT-PCR) and quantitative gene expression analysis (real-time-time PCR). Statistical analysis showed no difference in gene expression between the endometrium of women without endometriosis and endometrium from women with endometriosis. Showed a greater expression of the ID2 gene in advanced stage of endometriosis when compared to the initial stage and ovarian endometrioma compared to eutopic endometrium of women with endometriosis and PRELP in peritoneal lesion compared to eutopic endometrium of women with endometriosis. In the analysis performed with all lesions the SMOC2 was expressed more in the lesions (ovarian and peritoneal) compared to the eutopic endometrium of women with endometriosis. The results cited show that the expression of the genes studied may be influenced through the peritoneal cavity, and may be modified depending on the implantation site (ovary or peritoneum).

ASSUNTO(S)

endometriose endometriosis expressão gênica gene expression id2 id2 prelp prelp smoc2 and real-time pcr. smoc2 e pcr em tempo real.

ACESSO AO ARTIGO

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