Differential Accumulation of Proteinase Inhibitor I in Normal and Crown Gall Tissue of Tobacco, Tomato, and Potato 1

AUTOR(ES)

Wong, Peter P.

RESUMO

A proteinase inhibitor (inhibitor I) is induced in crown gall tumors of tobacco (Nicotiana tabacum) initiated through infection with the tumorinducing bacterium, Agrobacterium tumefaciens, strains B6 or CG-14. Uninfected tissues do not contain immunologically detectable quantities of inhibitor I. Inhibitor I synthesis in tobacco crown gall tumors paralleled tumor growth at the average rate of about 4.5 μg of inhibitor I per 200 mg of fresh tissue per day. Infection of variegated tobacco mutant Dp-I with A. tumefaciens strain CG-14 produced tumors with 25% more inhibitor than tumors induced with strain B6. Unlike tobacco, tumors induced by either bacterial strain on potato (Solanum tuberosum) and on tomato (Lycopersicum esculentum) did not accumulate inhibitor I. Consequently, inhibitor I accumulation is modulated by the type of plant host used in spite of familial relatedness (Solanaceae) and the strain of A. tumefaciens used for infection.

Documentos Relacionados

• Respiration and Phosphorylation of Mitochondria from Normal and Crown-Gall Tissue Cultures of Tomato 12
• Nonvolatile Organic Acids of Crown Galls, Crown Gall Tissue Cultures and Normal Stem Tissue. 12
• Oxidation of reduced diphosphopyridine nucleotide by mitochondria from normal & crown-gall tissue cultures of tomato 12
• Detection and Quantitation of Octopine in Normal Plant Tissue and in Crown Gall Tumors
• Nitrogen and Phosphorus Fractions, Respiration, and Structure of Normal and Crown Gall Tissues of Tomato