Diferenciação genética entre Melipona mondury, Smith 1863, Melipona rufiventris, Lepeletier, 1836 e Melipona sp. (Hymenoptera, Apidae) no estado de Minas Gerais, Brasil, utilizando marcadores ISSR / Genetic differentiation among Melipona mondury, Smith 1863, Melipona rufiventris, Lepeletier, 1836 and Melipona sp. (Hymenoptera, Apidae) in Minas Gerais, Brazil, using markers ISSR




Melipona rufiventris, Lepeletier, 1836 and M. mondury, Smith, 1863 (Hymenoptera, Apidae) are species genetically close popularly known as "uruçu amarela". Recent studies, using molecular markers showed that the population of "uruçu amarela" in Minas Gerais form distinct groups. M. mondury comes from the Atlantic Forest region, whereas M. rufiventris and a third species, known as Melipona sp. but not yet identified comes from the biome "Cerrado". The goal of this work was to study the genetic relationships between the population of "uruçu amarela" in the State of Minas Gerais using the ISSR molecular markers. 79 colonies from 10 different locations were used. The extracted DNA was amplified using nine ISSR primers and for the analysis one individual per colony was used. For the estimation of genetic differentiation the following analysis were done: (i) Percentage of polymorphic loci (P) and genetic diversity (ii) Genetic dissimilarity using the index of Dice and the method UPGMA (iii) Analysis of molecular variance (AMOVA) and φst. The findings showed a high polymorphism in molecular level among the colonies studied. In the grouping analysis the formation of three distinct groups, composed by M. mondury, M. rufiventris, Melipona sp.. We observed Melipona sp. the population of Urucuia showed significant genetic divergence in relation to the other samples.


issr meliponini hymenoptera variabilidade genética genetica issr meliponini genetic variability hymenoptera

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