Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
AUTOR(ES)
Lima, Lyana Rodrigues Pinto, Silva, Amanda Perse da, Schmidt-Chanasit, Jonas, Paula, Vanessa Salete de
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
16/02/2017
RESUMO
The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.
Documentos Relacionados
- A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
- Evaluation of Pre-Analytical Variables in the Quantification of Dengue Virus by Real-Time Polymerase Chain Reaction
- A Sensitive Quantification of HHV-6B by Real-time PCR
- Evaluation of the polymerase chain reaction for diagnosis of herpes simplex virus encephalitis.
- Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green
