Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.
AUTOR(ES)
Rotbart, H A
RESUMO
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=264111Documentos Relacionados
- Detection of Bordetella pertussis by rapid-cycle PCR and colorimetric microwell hybridization.
- Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
- Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection
- Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay.
- Detection and identification of aquatic birnaviruses by PCR assay.
