Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
AUTOR(ES)
Monte, Leonardo Garcia, Jorge, Sérgio, Luiz, João Paulo Mesquita, Sinnott, Francine, Seixas, Fabiana Kömmling, Aleixo, José Antonio Guimarães, Samartino, Luis Ernesto, Conceição, Fabricio Rochedo, Hartleben, Cláudia Pinho
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2012-06
RESUMO
Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
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