Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA.
AUTOR(ES)
Soares, M B
RESUMO
We have constructed a cDNA library from the mRNA of a rat liver cell line (BRL-3A) and characterized cDNA clones encoding the protein precursor of the rat insulin-like growth factor II (pre-pro-rIGF-II). This precursor, inferred from the nucleotide sequence, consists of a signal peptide, the rIGF-II sequence, and a trailer polypeptide of unknown significance. The characterized cDNA sequence (1016 nt) is part of a 3.4 kb mRNA species. Northern analysis reveals that a probe containing the extreme 5' noncoding region hybridizes to a second RNA (1.6 kb), while a probe corresponding to the 5' noncoding region proximal to the coding region hybridizes to two other RNA species (1.75 and 1.1 kb). All four RNAs are differentially expressed in all of the neonatal tissues that were examined, while the 3.4 kb pre-pro-rIGF-II mRNA and the 1.1 kb transcript are absent from adult tissues.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341060Documentos Relacionados
- Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5′ untranslated regions
- Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions.
- Cloning and tissue-specific expression of mouse macrophage colony-stimulating factor mRNA.
- Characterization and tissue-specific expression of the rat basic fibroblast growth factor antisense mRNA and protein
- Insulin, insulin-like growth factor II, and nerve growth factor effects on tubulin mRNA levels and neurite formation.
