Development of topical formulations containing papain to wounds treatment. / Desenvolvimento de formulações tópicas contendo papaína para o tratamento de feridas
AUTOR(ES)
Helaine Carneiro Capucho
DATA DE PUBLICAÇÃO
2007
RESUMO
The present work evaluated by content of protein measurement, electrophoresis, proteolytic activity determination and verification of this activity stability, raw materials of papain obtained from two manufacturers. Gel formulations of Carbopol® 940, Natrosol® 250 HHR and Pluronic® F127, and containing 1% of papain, were developed and evaluated with regard to stability and in vitro efficacy of proteolytic activity. The stability studies were conducted by papain proteolytic activity measurement, using casein as substrate and the samples were stored under different conditions of temperature and humidity. The in vitro efficacy of proteolytic activity was evaluated using polyacrylamide gel containing gelatin as substrate. The raw material obtained from manufacturer B showed higher content of protein and higher proteolytic activity than the one from manufacturer A. Both raw materials showed functional stability when stored at 4°C during 6 months. When stored at 30°C/70% RH and at 40°C/70% RH, there was significant loss of these activities. The measure of formulations proteolytic activity, after 48 hours from its preparation, showed that only 8% of the total activity was detected. This result might indicate possible interactions between papain and formulation components, which lead to a loss in the proteolytic activity or a reduction in the speed of enzymatic reaction. The functional stability studies showed that all formulations were stable when stored at 4°C during 6 months. However, when stored at 30°C/70%RH and 40°C/70%RH, it was observed fast decrease in the proteolytic activity as a function of storage time. The evaluation of formulations proteolytic activity in polyacrylamide gel, added with gelatin as substrate, after 6 hours of incubation at 37°C, showed that Carbopol® 940 gel formulation was the most efficient, being also better than the formulation recently prepared with 1% of papain raw material in aqueous solution. This result demonstrates that this polymer was the most adequate to be incorporated with papain. Besides this, it was the one that showed higher proteolytic efficacy in the in vitro test. Then, this formulation might be employed to help in the wound and burns cicatrisation process, in the different levels of health assistance, with efficacy, security and quality guarantee.
ASSUNTO(S)
papain proteolytic activity stability wounds estabilidade atividade proteolítica papaína feridas
Documentos Relacionados
- Development of topical formulations containing green propolis extract: stability, release, permeation and retention studies
- Development of electrochemical reactors for oil wastewater treatment.
- Desenvolvimento e avaliação de eficácia de formulações fotoprotetoras para a pele oleosa contendo extrato de Anacardium occidentale
- Desenvolvimento e avaliação clínica dos efeitos de formulações dermocosméticas contendo dimetilaminoetanol ou acetil hexapeptídeo-3
- TRATAMENTO DE EFLUENTES CONTENDO AMÔNIA