Development of Surface Plasmon Resonance-Based Immunosensor for Detection of Brucella melitensis

AUTOR(ES)

Saberi, Fatemeh, Kamali, Mehdi, Taheri, Ramezan A., Ramandi, Mahdi F., Bagdeli, Samira, Mirnejad, Reza

FONTE

J. Braz. Chem. Soc.

DATA DE PUBLICAÇÃO

2016-11

RESUMO

A label-free optical immunosensor was developed based on surface plasmon resonance (SPR) method for rapid and selective detection of Brucella melitensis. This immunosensor was constructed by immobilizing capture antibody on 11-mercaptoundecanoic acid modified gold disk. This fabricated immunosensor detected B. melitensis at a concentration range from 103 to 107 cell mL-1 (R2 = 0.998) and a detection limit of 100 cell mL-1. Additionally, the kinetic equilibrium dissociation constant (KD) for assessment of the interaction affinity was calculated as 1.1 × 10-9 mol L-1 that can be considered as high affinity interaction. This SPR immunosensor provided advantages in term of fast response, label free and accurate detection of B. melitensis in analytical systems.

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