Development of Molecularly Imprinted Polymers for Solid Phase Extraction of Parabens in Plasma Samples and Analysis by UHPLC-MS/MS
AUTOR(ES)
Roldão, Mariane V., Melo, Lidervan P., Miranda, Luis Felippe C., Resende, Marcos G., Queiroz, Maria Eugênia C.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2017-02
RESUMO
Two molecularly imprinted polymers (MIPs) were synthesized, by sol-gel and radical polymerization procedures. These MIPs were evaluated as selective stationary phases for miniaturized solid phase extraction (MISPE) to determine parabens in plasma samples by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Selective cavities of the MIPs, based on molecular recognition (hydrogen bonds), were revealed after removal of the template (benzylparaben). Both MIPs were characterized by scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). MIP prepared by radical process presented higher selective binding capacity for the target parabens than MIP prepared by sol-gel. Several variables that influence MISPE performance, including sample pH, sorbent washing, elution solvent, and elution solvent volume were also evaluated. According to the analytical validation parameters, the MISPE/UHPLC-MS/MS method is suitable for the analysis of parabens in plasma samples. The effectiveness of this method was demonstrated through the analyses of the umbilical plasma samples from postpartum women.
Documentos Relacionados
- Development and Validation of UHPLC-MS/MS for Simultaneous Determination of Scutellarin and Salicylic Acid in Rat Plasma: Application to Herb-Drug Pharmacokinetic Interaction Study
- Molecularly imprinted sol-gel silica for solid phase extraction of phenobarbital
- Evaluation of QuEChERS Sample Preparation for Determination of Avermectins Residues in Ovine Muscle by HPLC-FD and UHPLC-MS/MS
- DETERMINAÇÃO DE MULTIRRESÍDUOS DE AGROTÓXICOS EM MÉIS PRODUZIDOS NA REGIÃO DO TRIÂNGULO MINEIRO POR UHPLC-MS/MS
- Determination of Phenolic Compounds in Red Sweet Pepper (Capsicum annuum L.) Using a Modified QuEChERS Method and UHPLC-MS/MS Analysis and Its Relation to Antioxidant Activity