Development of a polymerase chain reaction-based diagnosis of Trichomonas vaginalis.
AUTOR(ES)
Riley, D E
RESUMO
We developed a polymerase chain reaction (PCR)-based test for detecting the protozoan parasite Trichomonas vaginalis. Genomic libraries were constructed from two independent clinical isolates of T. vaginalis. From these libraries, 12 genomic clones were purified, sequenced, and then screened for uniqueness by computer-assisted sequence comparisons. PCR reactions were performed to evaluate eight PCR-primer pairs, including a primer pair that targeted the T. vaginalis ferredoxin gene. All eight primer pairs yielded PCR products of the expected sizes. However, six of the primer pairs amplified their respective target sequences in limited numbers of clinical T. vaginalis isolates, suggesting the presence of significant genomic variability among isolates. An exception was a primer pair, termed TVA5-TVA6, that amplified a 102-bp genomic sequence, termed A6p, in all of 24 clinical isolates. The A6p sequence was not detected by PCR in human DNA or in a wide variety of flagellates, ciliates, or bacteria tested. The A6p sequence appears highly selective for a broad range of T. vaginalis isolates and holds promise for PCR-based diagnosis of the parasite.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265079Documentos Relacionados
- Polymerase chain reaction-based strain characterization of noncapsulate Haemophilus influenzae.
- Rapid, polymerase chain reaction-based identification assays for Candida species.
- Polymerase chain reaction-based detection of Trypanosoma cruzi DNA in serum.
- Polymerase chain reaction-Based clonality analysis of cutaneous B-cell lymphoproliferative processes
- A polymerase chain reaction-based method to detect cisplatin adducts in specific genes.