Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa.
AUTOR(ES)
Blumentals, I I
RESUMO
A two-step method is described for the production of exotoxin A by Pseudomonas aeruginosa in which a defined growth medium is modified for the toxin production phase. As a result, specific exotoxin A yields comparable to those obtained with complex media were achieved. In the development of this two-step process, several divalent metallic cations (Ca2+, Cu2+, and Mn2+), in addition to iron, were found to inhibit the yield of exotoxin A while Ca2+ and glycerol were found to increase yields. Northern blot analysis of total RNA isolates suggests that these effects on exotoxin A yields are based on events at the transcription level.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=204050Documentos Relacionados
- Two-step total gene synthesis method
- Development of a two-step enrichment procedure for recovery of Yersinia enterocolitica from food.
- An improved thermal cycle for two-step PCR-based targeted mutagenesis.
- Phagocytosis of unopsonized Pseudomonas aeruginosa by murine macrophages is a two-step process requiring glucose.
- The two-step to two-tier