Development and validation of an analytical method using High Performance Liquid Chromatography (HPLC) to determine ethyl butylacetylaminopropionate in topical repellent formulations
AUTOR(ES)
Pinto, Isadora Cabral, Cerqueira-Coutinho, Cristal, Freitas, Zaida Maria Faria de, Santos, Elisabete Pereira dos, Carmo, Flávia Almada do, Ricci Junior, Eduardo
FONTE
Braz. J. Pharm. Sci.
DATA DE PUBLICAÇÃO
22/06/2017
RESUMO
ABSTRACT Diseases caused by insects are frequent in poor countries, leading to epidemic scenarios in urban areas; e.g., Dengue, Zika and Chikungunya. For this reason, the development of a safe and efficient topical formulation is essential. Ethyl butylacetylaminopropionate (EB) is a mosquito repellent developed by Merck, which is used in products for adults, children and especially babies, due to its low allergenic potential. The aim of this work was to validate an analytical methodology to quantify EB in a new poloxamer-based formulation by high-performance liquid chromatography (HPLC). The quantification methodology was performed at 40 ºC using a Kromasil reverse-phase column (C18), with the dimensions of 250 x 4.6 mm. The mobile phase was acetonitrile:water (1:1) at a 1.0 mL/min flow-rate. The detector wavelength was set at 218 nm to detect EB. The methodology was considered validated since the results indicated linearity (R2>0.99), specificity, selectivity, precision and accuracy (active recovery between 98% and 102%). It also presented limits of detection and quantification of 0.255 µg/mL and 0.849 µg/mL, respectively. The present study demonstrated the EB vehiculated in poloxamer gel is promising as a new insect repellent formulation, since it could be quantified and quality control evaluated.
Documentos Relacionados
- Analysis of human plasma and urine purines using high performance liquid chromatography (HPLC)
- Development of an analytical method to quantify total isoflavones in phytotherapic capsules using high-performance liquid chromatography
- High-performance liquid chromatography (HPLC) assay for ribavirin and comparison of the HPLC assay with radioimmunoassay.
- Desenvolvimento e validação de metodologia para radiofármacos de Tecnécio- 99m empregando cromatografia líquida de alta eficiência (CLAE)
- Development and Validation of a High-Performance Liquid Chromatography Assay for Voriconazole