Development and Evaluation of a PCR Method for Detection of the Clostridium difficile Toxin B Gene in Stool Specimens
AUTOR(ES)
Guilbault, C.
FONTE
American Society for Microbiology
RESUMO
A PCR assay detecting Clostridium difficile toxin B gene in stool specimens was compared to the cytotoxicity assay as the reference standard for the diagnosis of C. difficile antibiotic-associated diarrhea (CDAD). Overall, 118 stool samples were tested. All of the specimens that were negative by the cytotoxicity assay (59 out of 118) were also negative by the PCR method (specificity of 100%). Of the 59 cytotoxin-positive samples, 54 were PCR positive (sensitivity of 91.5%). This PCR method is promising for rapid diagnosis of CDAD.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=130747Documentos Relacionados
- Evaluation of Two Rapid Assays for Detection of Clostridium difficile Toxin A in Stool Specimens
- Detection of vancomycin-resistant enterococci (VRE) in stool specimens submitted for Clostridium difficile toxin testing
- Fluorescent-antibody test for detection of Clostridium difficile in stool specimens.
- Latex agglutination test for detection of Clostridium difficile toxin in stool samples.
- Evaluation of Biosite Triage Clostridium difficile Panel for Rapid Detection of Clostridium difficile in Stool Samples