Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme-Linked Immunosorbent Assay†
AUTOR(ES)
Schoenthaler, S. L.
FONTE
American Society for Microbiology
RESUMO
We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 104 bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=95672Documentos Relacionados
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