Determination of the Carbon-Bound Electron Composition of Microbial Cells and Metabolites by Dichromate Oxidation
AUTOR(ES)
Harris, Robin F.
RESUMO
The applicability of the silver sulfate-acid dichromate oxidation (chemical oxygen demand) method for determining the carbon-bound electron compositions of microbial cells, substrates, and metabolic by-products was evaluated. An approach for approximating the carbon-bound electron composition of microbial cells from CHN data is also presented. Ten aliphatic and aromatic carboxylic acids, 17 amino acids, and 8 sugars generally gave 96 to 101% (mainly ≥98%) recovery with 0.0625 N dichromate (digestion mixture of 10 ml of sample-10 ml of 0.25 N dichromate-20 ml of Ag2SO4-amended concentrated H2SO4). Recoveries of nicotinic acid (5%) and methionine (65%) were incomplete; arginine (125%) and two purine and three pyrimidine bases (105 to 120%) were overestimated. The validity of 0.0625 N dichromate for determining the carbon-bound electron composition of bacterial cells was supported by theoretical analysis of the carbon-bound electron composition of hypothetical bacterial cell material (defined monomer composition) and by the compatibility of elemental and dichromate oxidation-derived carbon-bound electron compositions of typical bacterial cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243194Documentos Relacionados
- Soil Organic Carbon Determination Using NIRS: Evaluation of Dichromate Oxidation and Dry Combustion Analysis as Reference Methods in Multivariate Calibration
- Amino acids of the adrenal medulla and the effect of potassium dichromate oxidation on them.
- Composition of native and reconstituted chromatin particles: direct mass determination by scanning transmission electron microscopy.
- Humic Acids as Electron Acceptors for Anaerobic Microbial Oxidation of Vinyl Chloride and Dichloroethene
- Preparation of Metabolites of Spironolactone by Microbial Oxygenation
