Determination of rutin and narcissin in marigold extract and topical formulations by liquid chromatography: applicability in skin penetration studies
AUTOR(ES)
Fonseca, Yris Maria, Vicentini, Fabiana T. M. C., Catini, Carolina Dias, Fonseca, Maria José Vieira
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2010
RESUMO
A chromatographic technique for determination of rutin and narcissin in marigold extract and topical formulations was developed and validated. The method shows linearity over the concentration range of 0.2 - 6.0 μg/mL of rutin (r = 0.9986) and 0.8 - 12.0 μg/mL of narcissin (r = 0.9951). The values obtained for precision and accuracy are in agreement with ICH guidelines. Both the formulation excipients and the porcine ear skin samples did not interfere with the flavonoids determination. The recovery of rutin and narcissin in skin samples added with marigold extract was 81.41% and 83.35%, respectively, which demonstrate the applicability of this method to perform skin penetration studies.
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