Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry
AUTOR(ES)
Grecco, Caroline F.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2019-01
RESUMO
A reliable method was developed and validated based on dispersive liquid-liquid microextraction (DLLME) and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) to determine simultaneously methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), and butylparaben (BuPB) in breast milk samples. A central composite design 24 with four independent variables and five levels of variance was used to optimize the DLLME conditions. The DLLME/UHPLC-MS/MS method was linear from 5 (lower limit of quantification, LLOQ) to 100 ng mL-1 (upper limit of quantification, ULOQ). The inter-assay and intra-assay precision values presented coefficients of variation (CVs) lower than 15%, and the relative standard error of the inter- and intra-assay accuracy values was lower than 10.4%. The DLLME/UHPLC-MS/MS method was successfully applied to determine parabens in human milk samples obtained from sixteen volunteer lactating mothers.
Documentos Relacionados
- Determination of L-Ascorbic Acid in Milk by Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry Analysis
- Dispersive Liquid-Liquid Microextraction with Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Triazine, Neonicotinoid, Triazole and Imidazolinone Pesticides in Mineral Water Samples
- Analysis of chlorpheniramine in human urine samples using dispersive liquid-liquid microextraction combined with high-performance liquid chromatography
- Pharmacokinetics of oleracimine in rats by ultra-high-performance liquid chromatography
- Multi-Residue Method for Determination of Thirty-Five Pesticides, Pharmaceuticals and Personal Care Products in Water Using Ionic Liquid-Dispersive Liquid-Liquid Microextraction Combined with Liquid Chromatography-Tandem Mass Spectrometry