Determination of fipronil in bovine plasma by solid-phase extraction and liquid chromatography with ultraviolet detection

AUTOR(ES)

Cid, Yara P., Ferreira, Thais P., Medeiros, Deborah M. V. C., Oliveira, Rodrigo M., Silva, Nathália C. C., Magalhães, Viviane S., Scott, Fábio B.

FONTE

Quím. Nova

DATA DE PUBLICAÇÃO

2012

RESUMO

A fast and efficient method has been developed and validated for the determination of fipronil in bovine plasma. Samples were subjected to solid-phase extraction (SPE) followed by reversed phase liquid chromatography (LC) separation, using acetonitrile/water (60:40 v/v) as the mobile phase with a flow rate of 1.0 mL/min and ultraviolet (UV) detection at 210 nm. Ethiprole was used as the internal standard (IS). The method was found to be linear over the range 5-500 ng/mL (r = 0.999). The limit of quantitation (LOQ) was validated at 5 ng/mL. The method was successfully applied to monitor plasma concentrations following subcutaneous administration of fipronil in cattle.

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