Determination of Chlorophenol in Environmental Samples Using a Voltammetric Biosensor Based on Hybrid Nanocomposite
AUTOR(ES)
Mendes, Renata K., Arruda, Beatriz S., Souza, Elizabeth F. de, Nogueira, Alessandra B., Teschke, Omar, Bonugli, Luiz O., Etchegaray, Augusto
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2017-07
RESUMO
In this work, a simple electrochemical biosensor for 4-chlorophenol was developed based on laccase immobilized on a hybrid nanocomposite (ZnO nanoparticles/chitosan), and incorporated in a carbon paste electrode. There are few biosensors in the literature for this specific pollutant because it tends to form polymeric films on the electrode, causing surface passivation or even enzyme inactivation. The carbon paste allowed the surface to be easily renewed by polishing, which amends this limitation. To optimize the experimental conditions, we used cyclic voltammetry and hydroquinone as a representative of phenolic compounds due to the high toxicity of chlorophenol, thus avoiding the generation of hazardous residues. After optimization, a calibration curve was constructed for 4-chlorophenol using differential pulse voltammetry, and a linear response was obtained from 1 to 50 µM, with a lower detection limit of 0.7 µM. The obtained biosensor showed high accuracy when employed in the analysis of industrial wastewater.
Documentos Relacionados
- A biosensor based on immobilization of lactate oxidase in a PB-CTAB film for FIA determination of lactate in beer samples
- Detection of Anatoxin-a(s) in Environmental Samples of Cyanobacteria by Using a Biosensor with Engineered Acetylcholinesterases
- Preconcentration and determination of organochlorine pesticides in seawater samples using polyaniline/polypyrrole-cellulose nanocomposite-based solid phase extraction and gas chromatography-electron capture detection
- Modified electrode voltammetric sensors for trace metals in environmental samples
- L-ascorbic acid determination in pharmaceutical formulations using a biosensor based on carbon paste modified with crude extract of zucchini (Cucurbita pepo)
