DETERMINAÇÃO ESPECTROFOTOMÉTRICA DE METILDOPA EM ENSAIO DE DISSOLUÇÃO DE COMPRIMIDOS UTILIZANDO EXTRATO DE RABANETE COMO FONTE DE PEROXIDASE

AUTOR(ES)

Bueno, Natielle Gianine, Pereira, Airton Vicente

FONTE

Quím. Nova

DATA DE PUBLICAÇÃO

2015-09

RESUMO

An enzymatic spectrophotometric method for the determination of methyldopa in a dissolution test of tablets was developed using peroxidase from radish (Raphanus sativus). The enzyme was extracted from radish roots using a phosphate buffer of pH 6.5 and partially purified through centrifugation. The supernatant was used as a source of peroxidase. The methyldopachrome resulting from the oxidation of methyldopa catalyzed by peroxidase was monitored at 480 nm. The enzymatic activity was stable for a period of at least 25 days when the extract was stored at 4 or -20 ºC. The method was validated according to RDC 899 and ICH guidelines. The calibration graph was linear in the range 200-800 µg mL-1, with a correlation coefficient of 0.9992. The limits of detection and quantification in the dissolution medium were 36 and 120 µg mL-1, respectively. Recovery was greater than 98.9%. This method can be applied for the determination of methyldopa in dissolution tests of tablets without interference from the excipients.

Documentos Relacionados

• Determinação espectrofotométrica de metildopa em formulações farmacêuticas
• Sistema em fluxo para determinação espectrofotométrica de uréia em plasma de sangue animal empregando leguminosa como fonte natural da enzima urease
• Análise espectrofotométrica e visual do clareamento dental interno utilizando laser e calor como fonte catalisadora
• Determinação de nitrito em águas utilizando extrato de flores
• Determinação espectrofotométrica por injeção em fluxo de compostos fenólicos em águas residuárias empregando peroxidase de abobrinha (Cucurbita pepo)