DETERMINAÇÃO DE HIDROCARBONETOS POLICÍCLICOS AROMÁTICOS EM ÁGUAS SUPERFICIAIS NOS RIOS PIRACICABA E DOCE, IPATINGA MG, UTILIZANDO A TÉCNICA DE EFS-CLAE-DAD

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The polycyclic aromatic hydrocarbons belong to a group of organic substances which receive considerable attention because of the carcinogenic and mutagenic of some of them. They are released into the environment through natural and anthropogenic sources, and the anthropogenic shows greater contribution. However, in theory, for that process to be triggered it just need only a mutagenic action of a single molecule of carcinogenic substances. So important is the determination of PAHs in the environment in low concentration. In literature the methodologies for the determination of PAHs, mainly in water, providing the lowest limits of detection using the technique of liquid chromatography to high (HPLC) and detected by fluorescence, while the use of spectrophotometry in the ultraviolet visible involves the detection limits much higher, depending on the PAHs that you want to quantify. This work has developed an analytical methodology for the determination of the 16 PAHs indicated by the USEPA in samples of surface water by liquid chromatography with the detection by UV-Vis with array of diodes. This methodology resulted in a preconcentration of the sample of 1600 times using C18 cartridge to 200mg of solid phase. PAHs were extracted with dichloromethane and after drying on a stream of nitrogen, resuspended with 500μL of acetonitrile and separated by CLAE. A column C18 - LiChrospher PAHs (250mm x 3 mm, 5μm) was used and detection by UV-Vis with arrays of diodes (DAD) (F-20A Shimadzu SPD). With this method could be found, among other values, the detection limit of the order until 120pg/L for acenaftileno 1.31ng/L for benzo[a]pyrene. The validation of the method was performed with tests of accuracy, precision and recovery using environmental sample. For the construction of the analytical curve was used as internal standard that showed good linearity for concentrations between 5 and 400μg/L, corresponding to a range of concentration in the sample 3 to 250ng/L. The method developed here has proved efficient for the determination of PAHs in water and the results showed that the waters of rivers studied are contaminated with PAHs at levels fairly significant because some of the compounds, benzo[a]pyrene and chrysene, are found in concentrations higher than the accepted maximum limits set by the Council National Environment waters for the Class 1 and also suggest the issue of petrogênicas sources.

ASSUNTO(S)

1. hidrocarbonetos policíclicos aromáticos - teses. 2. cromatografia líquida - teses. 3. avaliação ambiental - minas gerais - teses. i. universidade federal de ouro preto. ii. título saneamento ambiental

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