Determinação de Cu, Fe, Mn, Zn e do teor de proteína total em amostras de trigo e soja após procedimento de extração sequencial
AUTOR(ES)
Bittencourt, Leila M., Lana, Diogo A. P. D., Augusti, Rodinei, Costa, Letícia M., Pimenta, Adriano M. C., Santos, Agenor V. dos, Gonçalves, Ana Paula F.
FONTE
Quím. Nova
DATA DE PUBLICAÇÃO
2012
RESUMO
A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L-1 NaCl, ethanol/water (70:30 v v-1) and 0.5 mol L-1 NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method.
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