Determinação da presença de anticorpos anti-espermatozoides no semen de casais submetidos a tecnicas de reprodução assistida

AUTOR(ES)

Ricardo Destro Saade

DATA DE PUBLICAÇÃO

1999

RESUMO

Antisperm antibodies (ASA) has been responsabilized as a well-stablished cause of male infertility. Conditions that disrupt the blood-testis barrier or changes in the female genital tract, could trigger its production. These ASA interfere in several steps of the fertilization, compromising the success of the assisted reproduction procedures. With the goal of identifying the presence of ASA in the semen of couples submitted to assisted reproduction techniques, as well as the antisperm immunoglobulin subtype involved and its location of binding on the spermatozoa surface, semen samples generated from two adult male groups, in fertile age, had been selected. The first group denominated normal, included 31 males with at least two children, whom were enrolled in a sterilization program. The second group included 30 male partners of couples submitted to assisted fertilization techniques (IUI/IVF). Each individual provided a semen sample which was submitted to a conventional spermogram and to ASA detection through the Direct Immunobead Test (Fertility Technologies, Inc.®). The ASA were detected in 10% of the individuals in the studied group, and in none of the controls; nevertheless, this difference was not statistically significant (p=0,113). The remaining analyzed variables, as concentration, motility and morphology had show significant differences between the groups (p=0,0258; p=0,012; p=0,0017; respectively). It, had been demonstrated solely the presence of A and G Immunoglobulins, and none of the Immunoglobulin M. Regarding the binding sites there had been no specifity neither to, local, nor to immunoglobulin subtype. In conclusion, the inclusion of the ASA analysis in the laboratory routine of infertile couples avaliation could identify the male immunologic factor and, hence, help choosing the most adequate assisted reproduction procedure (gamete micromanipulation)

ASSUNTO(S)

reprodução humana infecundidade masculina

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