Detection of viral sequences of low reiteration frequency by in situ hybridization.
AUTOR(ES)
Brahic, M
RESUMO
The sensitivity of in situ hybridization has been increased at least 10-fold by hybridizing in cDNA excess, by increasing the diffusion of the cDNA through the cells, by hybridizing at optimum temperature, and by stabilizing hybrids during autoradiography. Saturation of intracellular RNA with [3H]cDNA has been achieved. The assay is quantitative. In situ hybridization has been used to detect and quantitate visna virus RNA in infected cells. By using [3H]cDNA with specific activity of 2 X 10(8) dpm/micrograms and conditions that reduce background to negligible levels, 10--20 copies of viral RNA per cell can be detected and quantitated after 2 days of autoradiographic exposure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393131Documentos Relacionados
- Detection of Viral DNA Sequences in Adenovirus-Transformed Cells by In Situ Hybridization
- Detection of Viral DNA Sequences in Adenovirus-Transformed Cells by In Situ Hybridization
- Subcellular localization of low-abundance human immunodeficiency virus nucleic acid sequences visualized by fluorescence in situ hybridization.
- Rapid detection of human chromosome 21 aberrations by in situ hybridization.
- Cytomegalovirus in urine: detection of viral DNA by sandwich hybridization.
