Detection of Treponema denticola in Atherosclerotic Lesions
AUTOR(ES)
Okuda, Katsuji
FONTE
American Society for Microbiology
RESUMO
We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immunofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=87882Documentos Relacionados
- Effect of fluoride on Treponema denticola.
- Immunofluorescent staining for the detection of Treponema pallidum in early syphilitic lesions.
- Arginine catabolism by Treponema denticola.
- Characterization of fibrinolytic activities of Treponema denticola.
- Cytokine Responses to Treponema pectinovorum and Treponema denticola in Human Gingival Fibroblasts