Detection of ochratoxin A in porcine kidneys by a monoclonal antibody-based radioimmunoassay.
AUTOR(ES)
Rousseau, D M
RESUMO
By using an indirect enzyme-linked immunosorbent assay, eight monoclonal antibodies (MAbs) were selected. Mice were immunized with ochratoxin A that was conjugated to bovine serum albumin. The hybridoma cell line designated 10G2 was grown in tissue culture and as an ascites tumor. The MAb was characterized to be specific to ochratoxin A and of the immunoglobulin G (IgG) class. Subsequently, the ascites fluid of this hybridoma was used in a competitive solid-phase IgG radioimmunoassay on protein A-Sepharose CL-4B, with [14C]ochratoxin A as tracer. Porcine kidneys were extracted with 0.5% phosphoric acid in chloroform. A two-step cleanup was achieved on a Sep-Pak C18 cartridge and a Sep-Pak silica cartridge. Radioimmunoassay with MAbs coupled to protein A-Sepharose CL-4B allowed the detection of ochratoxin A in porcine kidneys at a concentration as low as 0.2 ng/g.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=203698Documentos Relacionados
- Diagnosis of Human Leptospirosis by Monoclonal Antibody-Based Antigen Detection in Urine
- Rapid detection of Bordetella pertussis by a monoclonal antibody-based colony blot assay.
- Cytokine detection by antibody-based proximity ligation
- Detection of rotavirus in stool specimens with monoclonal and polyclonal antibody-based assay systems.
- Serologic confirmation of Neisseria gonorrhoeae by monoclonal antibody-based coagglutination procedures.
