Detection of metalloproteinases of the gelatinases family and their inhibitors in the interpubic ligament of the mouse, during pregnancy / Detecção de metaloproteinases da familia das gelatinases e de seus inibidores no ligamento interpubico do camundongo, durante a prenhez

AUTOR(ES)

Renata Giardini Rosa

DATA DE PUBLICAÇÃO

2007

RESUMO

During pregnancy of some rodents a deep remodeling occurs in the pubic symphysis (PS). In the mouse, these fibrocartilagenousarticulation are gradually replaced by a dense connective tissue, forming the interpubic ligament (IL) at the end of pregnancy. Right after birth, this ligament is rapidly remodeled and the space between the two bones is quickly closed, five days after birth. It is well known that hormones such as relaxin, facilitates the growth and remodeling of organs in female reproductive tract, changing the regulation of biochemical processes that are involved in the remodeling of the extracellular matrix (ECM) of the uterus and cervix during pregnancy. However, little are known about pubic symphysis in when the subject is the involvement of metalloproteinases (MMPs) in this remodeling, as well as the participation of inflammatorycells that could be involved in the remodeling process of the mouse pubic symphysisduring pregnancy. In this study were used pubic symphysis of virgin mice and interpubic ligament in pregnant animais for the detection of MMPs in vivo and at in vitro (24h explants). MMPs from the gelatinase family (MMP-2 and MMP-9) were characterized by zymography and by immunohistochemistryessays, also MMP-8 (collagenase 2) that is believed to be associated almost exclusively with an inflammatory processoTransmission electron microscopy and light microscopy was also used associated with selectively staining, to evidence type of cells that could be involved in the pubic symphysis remodeling. Zymographydemonstratedthe presenceof gelatinases MMP-2 and MMP- 9 both in inactive and active forms, at the PS and IL. Immunohistochemistry,at the pubic symphysis and interpubic ligament, detected the expression of gelatinases and also MMP-8 in cells that were similar to fibroblasts. Morphological characterization evidenciated that cells that are similar to fibroblasts in the pubic symphysis of virgin animais are closely related to the pericellular region, being that during pregnancy,these spaces suffers an increase in this sub-compartment between cell and matrix. Qualitative and quantitative , analysis demonstratedlittle granulocytes in the pubic symphysis of virgin animais and in the interpubic ligament during pregnancy. Results that were obtained from this work support the hypothesis that the gelatinases could be involved in pubic symphysis remodeling through intrinsic processes, being evident that, both cellular phenotype modifications and also chemical modifications of the ECM, are fundamental to the rearrange of this articulation during mouse pregnancy, birth and pos-partum. The extensive remodeling of the ECM suggests hormonal role of the pregnancy in the modulation of the interaction between cell-matrix independently of an inflammatoryreaction in other organs during pregnancy

ASSUNTO(S)

matriz extracelular remodelação tecidual metalloproteinases extracellular matrix tissue remodeling metaloproteinase pubic symphysis sinfise pubiana

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