Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR

AUTOR(ES)

Magalhães, Kelly Grace, Passos, Liana Konovaloff Jannotti, Carvalho, Omar dos Santos

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2004-06

RESUMO

From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.

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