Detection of Cryptosporidium parvum in Horses: Thresholds of Acid-Fast Stain, Immunofluorescence Assay, and Flow Cytometry

AUTOR(ES)

Cole, D. J.

FONTE

American Society for Microbiology

RESUMO

Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (101 to 106/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 × 105 oocysts/g of feces for the IFA and AF techniques and 5 × 104 oocysts/g for flow cytometry.

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