Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.
AUTOR(ES)
Mohammed, N R
RESUMO
Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of chlamydial inclusions in McCoy cells is equal to that of Giemsa staining, toluidine blue staining is easier and faster to perform.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=498846Documentos Relacionados
- Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.
- Selective translocation of annexins during intracellular redistribution of Chlamydia trachomatis in HeLa and McCoy cells.
- Inhibition of Chlamydia trachomatis growth in McCoy, HeLa, and human prostate cells by zinc.
- Comparison of the sensitivity of primary isolation of Chlamydia. In irradiated McCoy cells or HeLa 229 cells treated with diethylaminoethyl-dextran (DEAE-D).
- Interaction of Chlamydia trachomatis organisms and HeLa 229 cells.