Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
AUTOR(ES)
Alves, Clebert José, Figueiredo, Salomão Moreira de, Azevedo, Sérgio Santos de, Clementino, Inácio José, Keid, Lara Borges, Vasconcellos, Sílvio Arruda, Batista, Carolina de Sousa Américo, Rocha, Vivianne Cambuí Mesquita, Higino, Severino Silvano
FONTE
Brazilian Journal of Microbiology
DATA DE PUBLICAÇÃO
2010-06
RESUMO
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
Documentos Relacionados
- Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil
- Pigmented Euglenophytes in a natural and shallow lake in the semiarid region of Paraíba State, Brazil
- Continental fishes from the Tambaba Environmentally Protected Area, Paraíba State, Brazil
- Background and Reference Values of Metals in Soils from Paraíba State, Brazil
- Helminths of the frog Pleurodema diplolister (Anura, Leiuperidae) from the Caatingain Pernambuco State, Northeast Brazil