Detection and Identification of Actinobacillus pleuropneumoniae Serotypes 1, 2, and 8 by Multiplex PCR
AUTOR(ES)
Schuchert, Jennifer A.
FONTE
American Society for Microbiology
RESUMO
Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=516343Documentos Relacionados
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