Detecção e quantificação de sementes de soja geneticamente modificada resistente ao glifosato em sistema hidroponico / Detection and quantification of genetically modified soybean seeds resistant to glyphosate in hydroponics system

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

This work aimed at to establish a detection protocol to genetically modified (GM) soybean seeds resistant to the glyphosate, characterize the symptoms of the seedlings, and verify the efficiency of the hidroponic cultivation in the detection and quantification of GM soybean seeds mixtures in conventional seeds samples. Five assays were performed to establish the detection protocol, using nutrient solution and herbicide solution in the concentrations of 0,0; 0,12; 0,24; 0,36 and 0,48% of the equivalent acid of the glyphosate and the periods that the seeds stayed in contact with the herbicide solution ware 24; 16; 8; 4 and 1 hour. The evaluated parameters were normal seedlings, seedling, root and hypocotyls length, and number of secondary roots. In the second study conventional seeds samples were tested added with 0; 1; 3 and 5% of GM soybean seeds. The recommended protocol consist in the seeds permanence in contact with herbicide solution to 0,12% of the e a of glyphosate, for four hours, followed by the transfer of the seeds for nutrient solution until completing five days. The parameters that best allow the differentiation of cultivar GM and non-GM are the seedling length and the presence of secondary roots. The hydroponics cultivation allows 100% of corrects results in the GM soybean detection, independent of the percentage of the contaminants in the sample and it presents accuracy in the quantification of the presence of up to 1% GM soybean seeds being its precision conditioned to the perceptual of present mixture in the sample.

ASSUNTO(S)

hidroponics soybean glycine max transgênicos hidroponia transgenics seeds melhoramento de plantas forrageiras e producao de sementes sementes soja glycine max

ACESSO AO ARTIGO

Documentos Relacionados