Detecção do Papilomavírus humano na cavidade bucal de pacientes com infecção por este vírus na mucosa cérvico-vaginal. / Human papillomavirus detection on oral cavity of patients with cervical mucosa HPV infection.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Human Papillomavirus (HPV) is the most frequent cause of sexual diseases. There are more than 100 different kinds of HPV and 24 have a close relationship to HPV oral infection. As yet, the route of infection to the oral cavity has not been established, nevertheless, selftransmission and close contact with infected individuals are considered the most probable ways of infection. The main aspects of this study were: 1) to determine the prevalence of oral HPV infection in women who have HPV infection in the cervical mucosa; 2) to detect anti-HPV immunoglobulin A in oral fluid and 3) to evaluate the risk factors for this virus infection. Samples of saliva and a mouth swab were colected in 100 patients and the presence of viral DNA in the oral epithelial cells was determined by polymerase chain reaction (PCR), using the primers (MY09-MY11). Indiret immunofluorencence was employed in the detection of IgA in saliva. Results show that 81% of oral epithelial cells samples were positive for DNA- HPV and 44% of oral fluid samples for IgA anti-HPV. This results of low specific IgA prevalence and high percentual of oral epithelial cells infected would be related with early oral HPV infection in some cases. It was also observed that the occurrence of cervical relapse injuries determined higher prevalence of IgA in the saliva. However, future studies will be necessary to better evaluate the immune response in HPV oral infection. Human Papillomavirus (HPV) is the most frequent cause of sexual diseases. There are more than 100 different kinds of HPV and 24 have a close relationship to HPV oral infection. As yet, the route of infection to the oral cavity has not been established, nevertheless, selftransmission and close contact with infected individuals are considered the most probable ways of infection. The main aspects of this study were: 1) to determine the prevalence of oral HPV infection in women who have HPV infection in the cervical mucosa; 2) to detect anti-HPV immunoglobulin A in oral fluid and 3) to evaluate the risk factors for this virus infection. Samples of saliva and a mouth swab were colected in 100 patients and the presence of viral DNA in the oral epithelial cells was determined by polymerase chain reaction (PCR), using the primers (MY09-MY11). Indiret immunofluorencence was employed in the detection of IgA in saliva. Results show that 81% of oral epithelial cells samples were positive for DNA- HPV and 44% of oral fluid samples for IgA anti-HPV. This results of low specific IgA prevalence and high percentual of oral epithelial cells infected would be related with early oral HPV infection in some cases. It was also observed that the occurrence of cervical relapse injuries determined higher prevalence of IgA in the saliva. However, future studies will be necessary to better evaluate the immune response in HPV oral infection.

ASSUNTO(S)

oral cavity cavidade bucal papilomavírus humano iga imunologia human papillomavirus iga

ACESSO AO ARTIGO

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