Detecção de rotavírus em amostras fecais de bovinos e água em propriedades rurais do vale do Paranhana, RS

AUTOR(ES)

Bianca Bergamaschi

FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

2012

RESUMO

The aim of present study was to assess a possible relationship between the contamination of water sources with animal husbandry in the region. In the localities belonging to Paranhana valley, the municipalities of Taquara, Rolante e Riozinho, were collected 104 water samples from different sources including tap water, ponds, dug wells, boreholes, streams, river and slopes. The water samplings were conducted in the vicinity of farms and on them. In addition, 38 fecal samples were collected from cattle dung from animals on propertie of dairy in Taquara.Water samples were subjected to a process of viral particles concentration and RNA extraction following standard methodology. For genomic RNA detection, were used reverse transcriptase followed by polymerase chain reaction (RT-PCR) targeting the most conserved gene of rotaviruses, gene encoded the viral protein 6 (VP6).To evaluate the presence of infective viruses samples were inoculated on MA-104 cells, HEp-2 and CRIB. Of all water samples, 25 (24%) contained genome of rotavirus, 8,66% samples from Rolante, 4,81% samples from Riozinho and 10, 59 samples collected on Taquara. The 25 samples containing viral genome which all were subsequently subjected to isolation in cultured cells, none were observed cytopathic effect typical of rotavirus (CPE). RNA extraction of these inoculations after three consecutive passages did not reveal the presence of rotavirus genomes. Regarding the stool samples, none were detected presence of rotavirus genome. Viral isolation, as used in this study did not have sufficient sensitivity to enable its use as a technique for rotavirus identification as water contamination.

ASSUNTO(S)

rotavirus rotavirus virologia veterinaria : bovinos water contaminação ambiental detection contaminacao : agua environmental contamination gastroenteritis

Documentos Relacionados

• Estudo de caso de dermatite causada por espécie de mariposa do gênero Hylesia (Lepidoptera: Heterocera) no Vale do Paranhana, RS
• Investigação do método de desenvolvimento de calçados no pólo calçadista do Vale do Rio dos Sinos e Paranhana, no Estado do Rio Grande do Sul
• Genótipos G e P de rotavírus em amostras fecais de crianças de Teresina, Estado do Piauí
• Detecção e classificação molecular de Chlamydophila psittaci em amostras fecais de aves assintomáticas
• Detecção simultânea de Coronavírus bovino e Rotavírus do grupo A em amostras fecais de bovinos utilizando uma multiplex hemi-nested RT-PCR