DETECÇÃO DE ESPÉCIES BACTERIANAS ENVOLVIDAS EM PROCESSOS DE BIODESSULFURIZAÇÃO EM UM SISTEMA DE LANDFARMING, NA REFINARIA GABRIEL PASSOS (REGAP), MINAS GERAIS.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The emission of sulfur from the burning of fossil fuels represents a global problem because it is the major cause of acid rain and air pollution. A major concern of the refineries is reducing the sulfur content of oil. The technology commonly used for this purpose is the hydrodesulfurization. An alternative that is being increasingly considered is biodesulfurization by using microorganisms to remove selectively the sulfur present in the hydrocarbon chains. Furthermore, this process can be performed in more mild conditions of temperature and pressure, which makes it less polluting and cheaper for the refinery. This work shows an isolation of bacterial strains capable of desulfurization from samples of soil contaminated with oil from a landfarming system in the Gabriel Passos Refinery (REGAP) in Minas Gerais. The strains were tested for their ability to use the compound dibenzotiophene using techniques as spectrophotometry and high performance liquid chromatography. Were also used molecular techniques such as extraction of DNA and BOX-PCR, in order to obtain a picture of the structure of microbial community present in the samples. We obtained 31 isolates with the ability to utilize DBT as a source of energy. Most of the isolates were considered as cocci and coccibacillus and 55% showed Gram-positive. With respect to growth curves, the isolates showed different patterns of growth among isolates with the same genomic profiles. The similarity analysis using UPGMA showed the formation of five groups with similar DNA patterns. Furthermore, we obtained at least two species or genera and possible new strains capable of desulfurization.

ASSUNTO(S)

biodesulfurization, dibenzothiophene, petroleum. saneamento ambiental biodessulfurização, dbt, petróleo.

ACESSO AO ARTIGO

Documentos Relacionados